All of our microscopes are housed in climate-controlled, black-out rooms. We specialise in live cell imaging, so each microscope has environmental controls to regulate temperature and CO2 levels for live cell imaging. Three of our microscopes also have oxygen control, for live hypoxia experiments.
Below are our basic setups, although custom solutions are available upon request. Please contact the facility manager for more information. The equipment mini-Posters (circa September 2017) are also available to download.
Which microscope for which experiment?
Type below to filter for microscope name, special features or techniques:
| Microscope Name | Illumination | Stand | Special Features | Use For |
|---|---|---|---|---|
| Cell Discoverer 7 BioSciences Hub |
LED; Lasers: 405, 488, 561, 640 |
Inverted | Incubation; CO2, Widefield; Laser scanning confocal; High resolution with Airyscan2 technology; |
High throughput; Automated platform; Autoimmersion and autofocus; Gentle imaging; 3D imaging |
| Elyra BioSciences Hub |
UV lamp; Lasers: 405, 488, 561, 642 |
Inverted | Incubation; CO2, Widefield; TIRF; Apotome mode; Lattice SIM; Superresolution: SMLM, PALM, STORM |
Fast Time Series; Superresolution; Gentle imaging; 3D imaging |
| Dragonfly BioSciences Hub |
UV lamp; Lasers: 405, 488, 561, 637, 730 |
Inverted | Incubation; CO2, O2 control; Widefield; Spinning disk confocal; TIRF; Superresolution: SRRF; Infrared imaging |
Fast Time Series; Superresolution; Gentle Imaging; Large Samples; 3D Imaging |
| 3i Spinning Disk Nuffield Hub |
LED; Lasers: 405, 445, 488, 514, 561, 635 |
Inverted | Incubation; CO2 control; Widefield; Spinning disk confocal; TIRF; FRAP; FRET; photo-activation |
Fast Time Series (50ms/frame); Gentle Imaging; Automated Live Cell Imaging; 3D Imaging |
| LSM 900 Airyscan2 - William Henry Duncan William Henry Duncan Building |
LED; Lasers: 405, 488, 561, 640 |
Inverted | Incubation; CO2 control; Widefield; Laser scanning confocal; High resolution with Airyscan2 technology; FRAP |
Superresolution; 3D Imaging |
| LSM 900 Airyscan2 Nuffield Hub |
LED; Lasers: 405, 488, 561, 640 |
Inverted | Incubation; CO2 control; Widefield; Laser scanning confocal; High resolution with Airyscan2 technology; FRAP |
Superresolution; 3D Imaging |
| LSM 880 BioAFM BioSciences Hub |
LED; Lasers: 405, 458, 488, 514, 561, 633 |
Inverted | Incubation; CO2, O2 control; Widefield; Laser scanning confocal; Spectral unmixing; AFM |
Subcellular Resolution; 3D imaging |
| LSM 880 Multiphoton BioSciences Hub |
UV lamp; Lasers: 405, 458, 488, 514, 561, 633, 1080 |
Upright | Incubation; CO2, O2 control; Widefield; Laser scanning confocal; Spectral unmixing; High resolution with Airy scan technology; Multiphoton |
Superresolution; Large Samples; 3D Imaging |
| LSM 800 Airyscan Nuffield Hub |
UV lamp; Lasers: 405, 488, 561, 640 |
Inverted | Incubation; CO2 control; Widefield; Laser scanning confocal; Spectral unmixing; High resolution with Airy scan technology; FRAP |
Superresolution; 3D Imaging |
| Leica SP5 Nuffield Hub |
LED; Lasers: 405, 458, 476, 488, 496, 514, 561, 594, 633 |
Upright | Widefield; Laser scanning confocal; |
AOBS (Acousto-Optical Beam Splitter); Large Samples; 3D Imaging |
| LSM 780 BioSciences Hub |
UV lamp; Lasers: FLIM: 405, 485, Confocal: 458, 488, 514, 561, 633 |
Inverted | Incubation; CO2, O2 control; Widefield; FRAP; FRET; FLIM; FCS; Spectral unmixing |
Subcelllar Resolution; 3D Imaging |
| Lightsheet Z.1 BioSciences Hub |
Lasers: 405, 488, 561, 638 | Light sheet | Incubation; CO2 control | Fast time series; Gentle Imaging; Large Samples; 3D Imaging |
| Widefield Nuffield Hub |
LED: 385, 470, 540-580, 625 | Inverted | Incubation; CO2 control; Widefield; FLIM; | Fast time series; Gentle Imaging; Optical Sectioning; DIC and PlasDIC |
| Epifluorescent BioSciences Hub |
UV lamp | Inverted | Incubation; CO2, O2 control; Widefield; DIC; Polarised light microscopy; Superresolution: SOFI; Microinjection |
Fast Time Series; Superresolution |
| Incucyte Nuffield Hub and BioSciences Hub |
LED: 488, 561 | Incubation; Phase contrast; Widefield; | Time Series; Medium throughput | |
| Histology BioSciences Hub |
UV lamp | Inverted | Widefield; Phase contrast microscopy |
Suppliers
Carl Zeiss
Carl Zeiss are a leading manufacturer of optics and microscope systems for a range of scientific disciplines.
Andor
We use Andor cameras for our high-speed / high sensitivity applications and have both EM-CCD and sCMOS cameras available. They also produce a number of systems for Active Illumination.
Picoquant
Our Lifetime imaging setup uses a Picoquant FLIm solution. We also have SymPhoTime64 available on several workstations for lifetime analysis.
Leica
Our Histology microscope is fitted with a Leica colour camera for histological or three-colour fluorescent imaging.
DTP
Most imaging work requires reliable high-end computer workstations for analysis and visualisation. DTP (based in Leeds) provides high-specification Hewlett-Packard workstations to the CCI.
© Liverpool Centre for Cell Imaging