Widefield

The Widefield is an epifluorescent microscope. Unlike the confocal microscopes, the epifluorescent uses Light Emitting Diodes (LEDs) to illuminate samples. The whole sample is illuminated with light of a specific wavelength, determined by an excitation filter. The light excites fluorescent molecules within the sample. Some of the emitted light passes through the objective and throough an emission filter, blocking the excitation light. The filtered transmitted light is visualised through eye pieces or captured by a camera.

Above, labelled cells were imaged ... . Data courtesy of ....

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