Elyra

Stand:

Zeiss Axio Observer 7

Laser Lines:

Objectives:

Other Features:

Application Notes:

The super-resolution PALM/Lattice SIM Elyra 7 is capable of fast and gentle imaging, as well as imaging well beyond the diffraction limit. It is a dual camera system built around a Zeiss Axio Observer 7 inverted microscope and specially designed for super-resolution microscopy techniques.

Several modalities are available:

1. Single-molecule localisation Microscopy, better-known by the two main techniques, Photoactivation and Localization Microscopy (PALM) and Stochastic Optical Reconstruction Microscopy (STORM).
2. Structured Illumination Microscopy (SIM). With this technique, images of twice the resolution of conventional microscopy in all three dimensions are possible (120 nm in xy and 300 nm in z). It uses a lattice (Lattice SIM) to achieve incredibly high-speed imaging at 255 fps. No specific sample preparation is required.
3. Apotome mode. Provides superfast optical sectioning for 3D samples.
4. TIRF. Optical sectioning of 100-200nm depth of the specimen from the glass surface.

Thanks to these modalities, the Elyra 7 allows the observation and characterisation of fastest dynamics in living biological samples in great detail for a wide range of fluorophores and length scales. The microscope stage is enclosed in an environmental control chamber for live-cell imaging (Temperature, CO₂, O₂).

Improvement in resolution from widefield to Lattice SIM. Multistained cells highlighting mitochondria (red), actin (green) and nucleus (blue).

The system was installed in December 2020 and will be launched at a 2021 CCI Mini-workshop. If you are interested in seeing the system or being trained to use it, please get in touch.

Publication Reference:

This piece of equipment was funded by BBSRC grant number BB/T017813/1. Please cite this grant code when publishing work conducted using this hardware.