Leica DMi8 with Andor Dragonfly
The Dragonfly from Andor Technologies is a cutting-edge microscope featuring a combination Total Internal Reflection Fluorescence (TIRF) and Spinning Disk confocal. This provides the advantages of optical sectioning with the speed of a camera-based system. The dual iXon 888 EM-CCD cameras can be used sequentially or simultaneously to speed up multi-channel acquisition.
The Dragonfly is capable of both far-red (~650-700nm) and infrared (700nm+) imaging, providing less photodamage and lower light scattering for better depth penetration.
The spectra above show typical green and red emission probes with an IR probe
(Data from ThermoFisher SpectraViewer).
The Dragonfly also includes real-time super-resolution imaging with SRRF-Stream technology. This allows imaging with of most common fluorophores and dyes down to ~80nm.
Focal adhesions are complex clusters of proteins and signalling molecules. Total Internal Reflection Fluorescence (TIRF) Microscopy drastically improves the image contrast as well as the resolution in the Z-plane. Here, TIRF images of DLC1 (red) and Talin (green) were imaged using inline SRRF processing.
Sample and imaging by Rebecca Kelly (Sée Lab).
By acquiring ~100 frames in quick succession, the intensity fluctuations can be correlated and analysed to produce a super-resolved image. Furthermore this can be done in any of the system's imaging modes: Widefield, TIRF or confocal.
This piece of equipment was funded by BBSRC grant number BB/R01390X/1. Please cite this grant code when publishing work conducted using this hardware.