Centre for Cell ImagingCCI

Dragonfly


Dragonfly

Stand:

Leica DMi8 with Andor Dragonfly

Laser Lines:

  • 405
  • 488
  • 561
  • 637
  • 737

Objectives:

Other Features:

Application Notes:

The Dragonfly from Andor Technologies is a cutting-edge microscope featuring a combination Total Internal Reflection Fluorescence (TIRF) and Spinning Disk confocal. This provides the advantages of optical sectioning with the speed of a camera-based system. The dual iXon 888 EM-CCD cameras can be used sequentially or simultaneously to speed up multi-channel acquisition.

The Dragonfly is capable of both far-red (~650-700nm) and infrared (700nm+) imaging, providing less photodamage and lower light scattering for better depth penetration.

The spectra above show typical green and red emission probes with an IR probe
(Data from ThermoFisher SpectraViewer).

The Dragonfly also includes real-time super-resolution imaging with SRRF-Stream technology. This allows imaging with of most common fluorophores and dyes down to ~80nm.

Two-colour SRRF in the nucleus of a cell. Sample provided by Jennifier Francis (Lévy Lab).

By acquiring ~100 frames in quick succession, the intensity fluctuations can be correlated and analysed to produce a super-resolved image. Furthermore this can be done in any of the system's imaging modes: Widefield, TIRF or confocal.

The system was installed in August and featured heavily at the 2018 CCI Imaging Workshop. If you are interested in seeing the system or being trained to use it, please get in touch.

Publication Reference:

This piece of equipment was funded by BBSRC grant number BB/R01390X/1. Please cite this grant code when publishing work conducted using this hardware.

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