Centre for Cell ImagingCCI

Lightsheet Z.1


Lightsheet Z1

Stand:

Zeiss Lightsheet Z.1

Laser Lines:

  • 405
  • 488
  • 561
  • 638

Objectives:

Other Features:

Application Notes:

Confocal Microscopes focus laser light into a point which is then scanned across the sample. Lightsheet (LS) imaging is gentler on the sample as only the fluorophores in the same plane as the focus are excited. As a further advantage, the z-resolution is improved due to a reduction in out-of-focus light.

Lightsheet microscopy allows imaging of relatively huge samples, such as adult Drosophila melanogaster about 1mm in length.

Spheroids, a model of tumor formation, are also highly amenable to this technique and allow visualisation of, for example, cell migration or metabolic gradients within tumours.

Publication Reference:

This piece of equipment was funded by BBSRC Alert13 grant number BB/L014947/1. Please cite this grant code when publishing work conducted using this hardware.

University of Liverpool

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