Zeiss Lightsheet Z.1
Confocal Microscopes focus laser light into a point which is then scanned across the sample. Lightsheet (LS) imaging is gentler on the sample as only the fluorophores in the same plane as the focus are excited. As a further advantage, the z-resolution is improved due to a reduction in out-of-focus light.
Lightsheet microscopy allows imaging of relatively huge samples, such as adult Drosophila melanogaster about 1mm in length.
Spheroids, a model of tumor formation, are also highly amenable to this technique and allow visualisation of, for example, cell migration or metabolic gradients within tumours.
This piece of equipment was funded by BBSRC Alert13 grant number BB/L014947/1. Please cite this grant code when publishing work conducted using this hardware.