Lightsheet Z.1

Confocal Microscopes focus laser light into a point which is then scanned across the sample. Lightsheet (LS) imaging is gentler on the sample as only the fluorophores in the same plane as the focus are excited. As a further advantage, the z-resolution is improved due to a reduction in out-of-focus light.

Lightsheet microscopy allows imaging up to the mesoscale, such the olive fly below, 5 mm in length.

Spheroids, a model of tumor formation, are also highly amenable to this technique and allow visualisation of, for example, cell migration or metabolic gradients within tumours.

Publication Reference:

This piece of equipment was funded by BBSRC Alert13 grant number BB/L014947/1. Please cite this grant code when publishing work conducted using this hardware.