Celldiscoverer 7 (Inverted)
The Zeiss Cell Discoverer 7 with integrated LSM 900 Airyscan Confocal is high throughput widefield and Laser Scanning Confocal with full environmental control and automation.
The setup also includes a Airy scan 2 detector providing superresolution images with Multiplex mode. Airyscan 2 in Multiplex mode can acquire up to eight superresolution image lines with a high signal to noise ratio. The LSM 900 provides high sensitivity and spectral flexibility.
The highest resolution objective is a water immersion objective with automated immersion medium dispensing function. For long-term, time-lapse imaging, the autoimmersion and automated focus detection capability ensure crisp contrast and high resolution images.
96 well plate imaged with differnt magnifications in three channels .
Above, labelled cells were imaged in widefield mode in a 96 well plate. The cells were labelled with a live (green) dead (magenta) staining kit to follow the progression of cell health during the course of an infection. Data courtesy of Dan Foulkes (Fernig lab).
Following pre-processing and analysis in Zen Blue 3.7, a heat map of measured parameters can be genearted. The heat map above shows the number of live cells per well over the course of the imaging time. There is a clear drop in cell numbers in rows A to D on the plate.
This piece of equipment was funded by MRC grant numbe MR/X013502/1. Please cite this grant code when publishing work conducted using this hardware.