Centre for Cell ImagingCCI

LSM 780


LSM780

Stand:

Zeiss Axio Observer Z.1

Laser Lines:

  • 440
  • 458
  • 488
  • 514
  • 561
  • 633

Objectives:

Other Features:

Application Notes:

Fluorescence is the mechanism by which energy (in the form of photons) is absorbed by a fluorophore then emitted at a longer wavelength. The characteristic time that a fluorphore exists in an excited state, before releasing a photon and returning to ground state, is called the Fluorescence Lifetime.

The lifetime is affected by many factors including the environmental conditions, non-radiative energy transfer and the presence of other flurophores.

As the lifetime depends both on radiative and non-radiative energy transfer, a measurable decrease in lifetime is seen during Förster Resonance Energy Transfer (FRET). Using this method allows for a rigorous and quantitative measurements of protein-protein interactions.

Publication Reference:

This piece of equipment was funded by MRC grant number MR/K015931/1. Please cite this grant code when publishing work conducted using this hardware.

University of Liverpool

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