2016 Imaging Workshop: Super-Resolution & Novel FLuorescent Proteins
Date & Location
The imaging day was held on the 21st and 22nd September 2016. The main seminars were in the North West Cancer Research Centre.
Program
Day 1 (21st Sept)
- 9:30-9:45 am: Introduction to the CCI - Violaine Sée & Daimark Bennett (CCI)
- 9:45-10:30 am: Foundation in microscopy - Dave Mason (CCI)
- 10:30-10:50 am: Coffee break
- 10:50- 11:35: Confocal and airyscan - Chris Power (Zeiss)
- 11:35-12:15: Fluorescent Lifetime Imaging - Chris Dunsby (Imperial College, London)
- 12:15-13:00: Selected short talks from users of the CCI
- 13:00-13:50: Lunch
- 13:50-14:30: Multiphoton and higher harmonic generation microscopy to visualize immune and cancer cell dynamics in live tissues - Bettina Weigelin (University of Texas MD Anderson Cancer Center , USA)
- 14:30-15:10: Smart fluorescent proteins and their application for high resolution microscopy - Peter Dedecker, (University of Leuven, Belgium)
- 15:10- 15:40: Coffee break
- 15:40-16:20: When one becomes two: Studying the biophysics of bacterial cell division with super-resolution microscopy - Seamus Holden (Newcastle University)
- 16:20-16:45: Cell segmentation and quantification - Delisa Ibanez-Garcia (Imaris)
- 16:45-16:50: Best short-talk prize announcement
By Appointment Only:
- 17:00-18:30: Hands-On Training with Imaris - please contact the facility if you're interested in attending this session as limited spaces are available.
Day 2 (22nd Sept)
- 10:00-5:00 pm: Meet the CCI staff, Zeiss specialists and Imaris Representitive with your samples and images. Compare your samples, fixed or living on different microscopes, get training on the new developments in data analysis and management with Imaris.
Registration
Registration is now closed for the 2016 CCI Imaging Workshop. Thank you to everyone who has registered.
Short Talks
Thank you to everyone who submitted an abstract. The following short talks were selected:
- Hayley Derricott (IGH): Developing a 3D intestinal epithelium model for livestock species
- Amelie Schober (IIB): USP29 is a non-canonical activator of hypoxia-inducible factor-α
- Megan Hasoon (IIB): Egg development plasticity and the transmission of maternal effects
- Lisa Luu (IGH): Developing an Alternative in vitro Model to study Enteric Pathogens
Our invited speakers awarded the best short talk prize to Megan Hasoon (Plaistow Lab). Congratulations on winning the iPad!
Invited Speakers
Peter Dedecker:
Peter's interests are the development of smart fluorescent proteins and their application for high resolution microscopy. He is one of the world leaders in Optical Fluctuation Imaging and an Assistant Professor at the University of Leuven.
Chris Dunsby:
Chris is a joint lecturer between the Photonics group, the Department of Physics and the Division of Experimental Medicine at Imperial College, London. His research interests are centred on the application of photonics and ultrafast laser technology to biomedical imaging.
Bettina Weigelin:
Bettina's research concentrates on understanding the interaction of immune and cancer cells with their microenvironment to identify and target cancer resistance niches during immunotherapy. To visualize the cellular dynamics within live tissues she uses intravital multiphoton microscopy and higher harmonic generation microscopy.
Seamus Holden:
Seamus' research centres on studying the biophysics of bacteria, especially during bacterial cell division, using single molecule fluorescence imaging, super resolution microscopy and spectroscopy.
Deliza Ibanez-Garcia:
Delisa has extensive experience in microscopy, multi-photon excitation and lifetime measurements (the topic of her PhD being FLIM in skeletal muscle). In 2009 she joined Bitplane, working as a sales representative and image analysis specialist.
Chris Power:
Chris is a 3D Imaging Specialist at Carl Zeiss UK. As project manager he has overseen the development of the Zeiss Lightsheet Z1 from optical bench to market and continues to support users of this and other technologies.
Sponsors
The CCI wishes to thank the following companies for sponsoring the Workshop (click for links):
© Liverpool Centre for Cell Imaging