2014 Imaging Workshop: Focus On Lightsheet Microscopy
Date & Location
The imaging day was held on the 13th and 14th October 2014. The main seminars were held in the North West Cancer Research Centre.
Day 1: Making the most of Microscopy
- 9:20-9:30 am: Welcoming Remarks (CCI)
- 9:30-11:00 am: Basic training talks on microscopy including user-suggested topics such as choice of microscope, deconvolution, image formation, spectral un-mixing - Andy Barlow, Chris Hudson and Chris Power (Zeiss)
- 11:00-11:20 am: COFFEE BREAK
- 11:20-11:50 am: Fast & Sensitive Detectors for a Range of Microscopy Applications - Orla Hanrahan (Andor).
- 11:50-1:10 pm: 5 selected short talks by PhD students and post-doc from users of the CCI
- 1:10-2:00 pm: LUNCH
- 2:00-3:00 pm: Use of OMERO for image data management and storage - Colin Blackburn (Dundee)
- 3:00-3:30 pm: Image analysis using Fiji - Dave Mason (CCI)
- 3:30-3:45 pm: Best short-talk prize announcement and transfer to computer room for hands on session
- 3:45-5:00 pm: Hands on workshop on use of OMERO - Colin Blackburn and June Matthew (Dundee)
Day 2: Lightsheet Microscopy for Beginners
- 9:30-10:00 am: Introduction to Light sheet microscopy - Chris power (Zeiss)
- 10:00-11:00 am: All you need to know about sample preparation for light sheet microscopy - Marco Marcello (CCI) and Chris Power (Zeiss)
- 11:00-11:30 am: COFFEE BREAK
- 11:30-12:15 pm: Light sheet imaging of arabidopsis - Daniel Von Wangenheim (Stelzer lab, Frankfurt)
- 12:15-12:45 pm: Light sheet microscopy and mathematical modelling - Stephanie Höhn (Goldstein lab, Cambridge)
- 12:45-1:00 pm: Deeper, faster and gentler on the sample: some novel applications of Lightsheet microscopy - Marco Marcello (CCI)
- 1:00-2:00 pm: LUNCH
- 2:00-4:30 pm: Demo of the light sheet microscope and practical on sample preparation - Chris Power (Zeiss) and Marco Marcello (CCI)
Registration is now closed for the 2014 CCI Imaging Workshop. Thank you to everyone who has registered.
Stephanie is a member of the Department of Applied Mathematics and Theoretical Physics (DAMTP) in the research group of Ray Goldstein at the University of Cambridge, UK. Her current research focuses on the biological and biophysical aspects of development and embryonic cell sheet curling in Volvox and its relatives.
Daniel Von Wangenheim:
Daniel is a member of Ernst Stelzer's lab at the Buchmann Institute for Molecular Life Sciences in Frankfurt. He uses Lightsheet microscopy to study the dynamics of plant growth at organ, cell and sub-cellular levels.
Orla has worked with Andor Technology as an Application Specialist in Life Science for the past four years. Her role brings her in touch with all the latest developments and innovations in both camera technology and microscopy applications.
Chris is a 3D Imaging Specialist at Carl Zeiss UK. As project manager he has overseen the development of the Zeiss Lightsheet Z1 from optical bench to market and continues to support users of this and other technologies.