2017 Imaging Workshop: Making the most of Microscopy
We're happy to announce the tentative program for the 2017 CCI Imaging Workshop. Our aim is to provide existing and potential users of the facility with a better idea of the services we offer, the work that is conducted here and some of the cutting edge science happening in the Imaging field.
We are pleased to have special guest Keynotes from Gary Borisy (Forsyth Institute) and Teng-Leong Chew from the HHMI Janelia Advanced Imaging Centre (AIC) who will also provide more in depth training on days two and three.
As with last year, we (and others) will be tweeting leading up to the event and in real time with the hashtag #CCIwkshp.
Date & Location
The imaging workshop was held on the 11th - 13th September 2017. The main seminars were held in the School of Life Sciences Lecture Theatre #2. The hands-on was held in the Life Sciences PC teaching room.
Program
Day 1
- 9:30-9:45: Introduction to the CCI - Violaine Sée & Daimark Bennett (CCI)
- 9:45-10:30: Foundation in microscopy - Dave Mason (CCI)
- 10:30-11:00: Choosing the Right Fluorophores - Marco Marcello (CCI)
- 11:00-11:15: Addgene: The non-profit plasmid repository - Benoit Giquel (Addgene)
- 11:15-11:35: Coffee break
- 11:35- 12:05: Experimental design (filters, mirror, dichroic, what to choose?) - Chris Power (Zeiss)
- 12:05-12:50: Selected short talks from users of the CCI. See below for details.
- 12:50-13:40: Lunch
- 13:40-14:25: Keynote #1: Mapping Microbiomes at the Micron Scale - Gary Borisy (Forsyth Institute, USA)
- 14:25-14:45: Bacterial behaviour on modified silicon nanowire arrays - Yuri Diaz Fernandez (University of Liverpool)
- 14:45- 15:10: Arivis for Visualisation and Analysis - Tamara Manuelian (Arivis AG)
- 15:10- 15:30: Coffee break
- 15:30-16:30: Keynote #2: Imaging Life with the Emerging Frontiers in Microscopy - Teng-Leong Chew (Advanced Imaging Centre - HHMI Janielia Research Campus)
- 16:30-16:40: Best short-talk prize announcement and closing remarks
Day 2 & 3
The director of the Advanced Imaging Centre at the HHMI Janelia Research Campus, Teng-Leong Chew gave a comprehensive hands-on training session on Image Analysis. The program covered:
Day 2
- Introduction to Digital Images - Fundamentals of digital images, file format, color scheme
- Fundamentals of Image Processing - Histograms, displays, pixel adjustment, filters, kernels and structuring elements
- Fourier Transformation - Image processing and denoising in Fourier space
- Object Segmentation - Turning pixel map into discrete objects for image analysis
Day 3
- Object-based Colocalization Analysis - Quantification of overlapped objects
- Pixel-based Colocalization and Intensity Analysis - Intensity measurement, interpretation of colocalization coefficients, ratiometric imaging
- Analysis to track changes and movement - Fluorescence recovery rate, particle tracking, kymograph, motion analysis
Registration
Registration for the 2017 Imaging Workshop is open until 4th September. There are a limited number of spaces and registration will be on a first-come first serve basis. Registration is free but required so that we can calculate catering and space requirements.
Registration is now closed for the 2017 CCI Imaging Workshop. Thank you to everyone who has registered, we look forward to seeing you on the 11th September.
Short Talks
Thanks to everyone who submitted an abstract. The following (10 minute) short talks were selected:
- Laetitia Petit-Jentreau (IGH): Integrin-mediated regulation of NK cell motility and function
- Marie Held (IIB): Clearing mouse tissue for light microscopy imaging
- Luke Johnson (IGH): Developing 3D in vitro disease models of the small intestinal epithelium and intestinal DCs for Toxoplasma gondii infections
- Fang Huang (IIB): Identification of a regulator essential for carboxysome formation in cyanobacteria
Congratulations to Fang Huang (Liu Group) who won the best short-talk prize, an iPad Mini!
Invited Speakers
Teng-Leong Chew:
Leong is Director for the Advanced Imaging Center at the HHMI Janelia Research Campus. Here, he leads the effort in building the unique collaborative imaging center that serves as the gateway through which the wider scientific world can access Janelia’s cutting-edge microscopy capabilities.
Gary Borisy:
Gary is a former president and director of the Marine Biological Laboratory in Woods Hole, Massachusetts. He is well know for his work on the cytoskeleton however, since joining the Department of Microbiology at the Forsyth Institute he has focused on imaging microbial comunities using advanced imaging techniques.
Tamara Manuelian:
Tamara is Application Specialist and Sales Manager for Imaging and Image Analysis software solutions at arivis AG, Germany. She is trained as a biologist and has long-term professional experience in software-related project and solution development, consulting and project management.
Benoit Giquel:
Benoit is an outreach scientist at Addgene, the non-profit plasmids repository. His role is to raise awareness about Addgene in Europe and to help European scientists sharing their plasmids with the international research community.
Chris Power:
Chris is a 3D Imaging Specialist at Carl Zeiss UK. As project manager he has overseen the development of the Zeiss Lightsheet Z1 from optical bench to market and continues to support users of this and other technologies.
Sponsors
The CCI wishes to thank the following companies for sponsoring the Workshop (click for links):
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